The fabrication of suitable porous polymer scaffolds, which mimics natural tissues, is one of the main challenges of tissue engineering. Bacterial nanocellulose (BNC) hydrogel resembles the native extracellular matrix (EMC) and the immobilization of bioactive molecules into the hydrogel 3D microstructure could be favorable to support cell adhesion and proliferation. The main goal of this work was the functionalization of BNC hydrogel with IKVAV peptide to produce scaffolds for tissue engineering and regenerative medicine (TERM) applications. Our results showed that BNC-IKVAV hydrogel is a promising material for TERM applications.