This work examines the performance of a distillation column using a solution of ethanol and water as a feed. The limits for the concentration and flow rate of the feed solution in the column were established to be 5-9% by volume and 1848-4385 g.h-1, respectively. Eleven tests were performed, each with three samples. Statistical analysis was performed to assess if the independent variables influenced the production of ethanol in accordance with Brazilian legislation, i.e., a Hydrous Ethanol Fuel with an alcohol content between 92.5 and 93.8% by mass. The concentration and feed flow rate were significant for the distillate and the recovery ratio of ethanol were above 80% in 10 of 11 tests, demonstrating that the performance of the column is satisfactory.