The components of fusel oil form azeotropes with water, resulting in challenges in obtaining hydrous ethanol fuel (HEF) as well as higher investment and operating costs. The aim of this study was to evaluate the influence of fusel oil during HEF distillation. Mixtures of ethanol (6.0 to 6.5 %w/w) and water (93.0 to 94 %w/w) containing different concentrations (zero to 0.5 %w/w) of 3-methyl-1-butanol, 2-methyl-1-propanol, propan-1-ol and propan-2-ol were distilled in a laboratory apparatus. The results obtained in total reflux demonstrated the possibility of obtaining HEF according to Brazilian legislation specifications, although the desired concentration was not reached during continuous operation. All experiments resulted in a high loss of ethanol in the bottom product across multiple operating conditions. The results demonstrated that while fusel oil does affect HEF distillation, it does not prevent the attainment of HEF that is in conformity with Brazilian legislation.