Solid wastes from agro-industries may be used as culture medium for enzymes synthesis by filamentous fungi. This work aims to extract and quantify the lipid presents in brewer’s spent grain. The lipids extraction was carried out by Soxhlet extraction method. Initially, the residue was maintained at 80°C to remove the moisture. After, a sample of 5 grams was weighed. A flat-bottomed flask, containing some glass beads, was dried at 80°C and it was weighed. The sample was introduced into the extractor unit in a sealed filter paper envelope. The solvent was added to flat-bottomed flask. The scheme was maintained on a hot plate for 4 hours. After, flat-bottomed flask was maintained at 80°C for the complete solvent evaporation. Then, the flask was weighed again, to determinate the lipids mass. We can conclude that this study provides the basis for further studies, since the percentage of lipids present in this residue (8.6%) justifies its use in experimental tests that aim lipase production.