The removal of organic contaminants from water and wastewater is a key problem of environmental remediation which can be solved by adsorption process, especially with the use of organoclays for their abundance, variety and low cost. Therefore, this study aimed the evaluation of modifications on the structure of a commercial bentonite organoclay named Spectrogel due to the adsorption process with gasoline. The characterization of the organoclay was accomplished through TGA, DSC, FT-IR, SEM/EDX, XRD and Helium picnometry analyzes before and after the process of gasoline and iso-octane removal. Modifications in the organoclay structure were verified and could be attributed to the adsorption process of the organic compounds.