12th International Conference of Pharmaceutical Sciences

Medicinal plants are important sources of bioactive constituents in the search for innovative therapeutic agents. The treatment of dermal lesions (wounds, ulcerations, etc.) still has few effective agents. The species under study is a plant present in the Amazon, popularly used for the treatment of bruises, being recently evidenced by our group that presents anti-inflammatory and analgesic effects through preclinical trials. Thus, the present work aimed to investigate the effects of the aqueous extract of its leaves, identified here as C002, on cell viability of cultured fibroblasts, the cicatrization process in vitro and possible antibacterial activity. BALB / c 3T3 murine fibroblasts were used for cell culture assays. In the cell viability test, the culture was exposed to increasing concentrations of C002 (15,625; 31,125; 62,25;125; 250; 500 µg/ml), being evaluated after 24h or 48h for cell survival. For evaluation of in cicatrization activity, homogeneous lesions were mimicked in the fibroblast monolayer and then treated with C002 at concentrations of 31.125; 62.25; 125 µg / mL or SBF (control). The images of the lesions, taken by optical microscope at 0, 24, 48 and 72 hours from the lesion, the area of the lesion was verified through the ImageJ® program. The evaluation of antimicrobial activity by microdilution against S. aureus, E. fecalis, E. coli and P. aeroginosa bacteria. The data obtained showed that C002 does not cause damage to the viability of fibroblasts in any of the tested concentrations within 24h. In the 48h interval, only the highest concentration tested (500 µg / ml) reduced cell viability. In the analysis of the healing process, the treatment of lesion C002 (62.25 µg / mL) promoted significant benefits, accelerating the regression of the lesion from the first 24h, showing about 25% superior to the control. Full recovery was observed at 48h after injury induction, while control only at 72h. It was also possible to verify that C002 is devoid of antimicrobial action against the bacteria tested. Thus, it was possible to verify that C002 has low cytotoxicity and promising potential for the treatment of dermal lesions, and further studies are necessary to elucidate its mechanisms of action.