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Erythromycin is a macrolide antimicrobial which acts by interfering with bacterial protein synthesis. This drug has a wide activity against gram-positive bacteria, such as streptococci, staphylococci and diplococci. Its use in fish farming is not regulated in Brazil yet, although it is approved in other countries. A study of drug incorporation in the feed with colorless and flavorless gelatin has been carried out aiming a homogenous process, avoiding or mitigating the risk of leaching of the drug from the feed to the aquatic environment. The method showed an incorporation efficiency of 70%, an extraction efficiency of 100% and resulted in a homogenous fish feed CV = 2% (n = 6). On the leaching test, in 15 min only 1% of erythromycin leached into the water. Analyzes were performed employing liquid chromatography associated with mass spectrometry. The main objective is to carry out a residual depletion study of erythromycin in fish after oral administration.
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