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Bothrops moojeni (Hoge, 1966) is a common terrestrial snake, of the family Viperidae and is found in South America, and throughout the Cerrado domain in central and southeastern Brazil in open habitats, almost always associated to gallery forests. This species has a very varied diet, composed of amphibians, lizards and rodents, and there is an ongeneous change in their diet. During the herpetological survey in January 2017 at RPPN Cisalpina, a conservation unit located in the municipality of Brasilândia in the Mato Grosso do Sul state, we collected a B. moojeni adult specimen which was necropsied and its stomach contents examined. We found an adult frog, Pithecopus azureus (Cope, 1862) (Anura: Hylidae) and an adult lizard Ameiva ameiva (Linnaeus, 1758) (Squamata: Teiidae). This is the first record of P. azureus and A. ameiva as food item of B. moojeni. In most studies about diet of this species, the identification of food items is given at class level, thus, this study contributes to the knowledge of the species used as food by this snake species.