The genus Croton belongs to Euphorbiaceae family and has been widely investigated due to its therapeutic uses and the large number of bioactive compounds obtained from its species1. Of the 312 species cataloged in Brazil2, Croton floribundus, popularly known as Capixinguí, stands out due to its widespread use in folk medicine, as in wound healing3 , and in the fight against syphilis and hemorrhoids4. It is also used to treat eye diseases, including trachoma, and its bark is strongly purgative5. Thus, this work describes the isolation and characterization of a clerodanic diterpenoid from this medicinal species. The collected vegetal material (leaf, stem and root) was submitted to the 95% hydroethanolic overnight extraction and after rotaevaporated, the aqueous fractions were submitted to solid phase extraction through C18 cartridges, resulting in fractions with different polarities. These fractions were analyzed by HPLC-DAD and HPLC-MS and the 80% fraction of the root was promising. The purification step of this fraction was done by preparative RP-HPLC using linear acetonitrile gradients. Pure compound CF.R.2 was characterized by 1D (1H, 13C and DEPT-135) and 2D (COSY, HMQC, HMBC, TOCSY and NOESY) Nuclear Magnetic Resonance spectroscopic techniques and HRMS. In conclusion was characterized a novel clerodane diterpenoid with spiro-γ-lactone group at C-9 substituted at C-12 with a furan ring.