Optimization of especial metabolites extraction parameters, phytochemical profile obtained by GC-MS, content of total phenols and flavonoids, and antioxidant activity of leaves extract from Sebastiania macrocarpa Mull Arg.

Vol 9, 2023 - 166377
Poster
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Abstract

This work aimed to develop a UHPLC-DAD chromatographic method for S. macrocarpa leaf extract to optimize extraction parameters for especial metabolites. The GC-MS analysis identified 20 compounds; optimized extraction: 20 mg/mL, 60% ethanol, 45 min. Resulting lyophilized extract yield: 26%. Total phenolic content: 935 μg GAE/g, flavonoid content: 196 μg QUE/g. Antioxidant activity was assessed via ABTS and DPPH assays: 0.120 and 0.158 μM Trolox/g, respectively

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Institutions
  • 1 Universidade Federal do Rio Grande do Norte
Track
  • 1. Biodiversity and Natural Products
Keywords
Sebastiania macrocarpa; UHPLC optimization; Phytochemical Profile; Antioxidant