Molecular Networking for Identification of Cytochalasins from Endophytic Fungus Preussia sp.

The dereplication of fungi extracts of Preussia sp. was performed by GNPS using UPLC-QTOF/MS data. It is reported here in the putative annotation of the cytochalasin cluster. The precursor ions [M + H]+ at m/z 452, [M + H]+ at m/z 480 and [M + H]+ at m/z 466 showed a neutral loss of 18 Da, suggesting that the compounds are cytochalasin J, cytochalasin B and ascochalasin, respectively. The Czapek extract (76%) showed a high percentage of inhibition against the protozoan Leishmania infantum.