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The economic development of nations is fully attached to how they use sources of energy. In 2016, the consumption in the industrial refrigeration sector was 51%. Traditional refrigeration systems use natural refrigerators such as water, air, hydrocarbons, ethylene glycol, etc. A viable alternative that aims to improve the properties of fluids is adding nanoparticles (NP) dispersion in those fluids, getting a colloidal sol called nanofluid (NF). We present obtaining NPs produced from the synthesis and modification of copper oxide functionalized with glycine using factorial design for application as an NF. These materials were obtained from the chemical reduction method, and for the second step, the oxidation with the finality of obtaining copper oxide NPs. To receive the first composite samples and adjusted synthesis conditions with the aim of getting copper metallic nanoparticles functionalized were proposed as a factorial design 2^3. The obtained samples were featured by XRD to identify the crystalline phases and FTIR spectra to assign chemical composition to the samples. The ascorbic acid factor did not affect the diameter of the nanoparticles formed, changing the factorial design into 2^2. It was possible to observe that the NP with the smallest diameter is related to less glycine mass and less copper precursor mass, which were considered critical factors. Preliminary results indicate the formation of copper oxide nanoparticles with an average crystallite size of an approximated 36.11 nm using Scherrer’s equation, in which its characteristic peak was observed at 35.4 degrees.
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