qNMR APPROACH FOR MONITORING CHANGES IN CARANHA FISH POLAR AND NON-POLAR METABOLIC PROFILES DUE TO FREEZE-THAW CYCLES

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Detalhes
  • Tipo de apresentação: Poster Presentation
  • Eixo temático:
  • Palavras chaves: fish meat quality; Metabolic profile; qNMR;

Autoras(es):

  • 1 Federal Institute Goiano
  • 2 Institute of Chemistry, Federal University of Goias
  • 3 Federal Institute of Goiás

qNMR APPROACH FOR MONITORING CHANGES IN CARANHA FISH POLAR AND NON-POLAR METABOLIC PROFILES DUE TO FREEZE-THAW CYCLES

Vinícius Silva Pinto

Universidade Tecnológica Federal do Paraná, Ponta Grossa

Resumo

Fish is considered an important source of polyunsaturated fatty acids, essential amino acids and bioactive components such as vitamins and osmolytes. The availability of these compounds is maximum in fresh fish. However, the high humidity and the pH close to neutrality make the fish a product with a short shelf life [1]. To delay the depreciation of quality, the use of cold is indicated in all stages of processing, characterizing the cold chain [2]. Once interrupted, unwanted reactions are initiated and nutritional and texture changes can occur, compromising its consumption [3]. While these changes are important, it is not always possible for the consumer to differentiate refrozen fish from fresh fish due to the similarity when exposed to ice. In this context, the present study was to evaluate the applicability of quantitative NMR combined with chemometrics to characterize and differentiate fresh (control group) and refrozen (treatment group) of Caranha fish meat. chemical descriptors related to endogenous and exogenous enzymatic activities in both extraction phases (polar and non-polar) were identified, indicating an increase in the degradative action during the refreezing cycles. Changes in the concentrations of trimethylamine, dimethylamine, inosine, hypoxanthine, lactic acid, taurine, creatine, polyunsaturated fatty acids and triacylglycerides were associated with a decrease in the quality of fish meat when compared to fresh samples.

Questões (2 tópicos)

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Autor

Vinícius Silva Pinto

Lorena, thank you for your kind message.
I used ERETIC 2. The standards used were maleic acid in D2O and caffeine in CDCl3.

Lorena Mara Alexandre e Silva

Nice. Thanks for your feedback. Cheers

Autor

Vinícius Silva Pinto

Dear Elenilson, thank you for paying attention to my presentation.

Yes, all the work was done by me. Initially, I performed an exploratory analysis to actually identify whether metabolic profiles were influenced by cold chain breakage. From this, I determined the T1 via t1ir1d (pulse sequence) for all metabolites responsible for the discrimination and compared them with the T1 value of maleic acid (D2O) and caffeine (CDCl3), used as calibration standards for ERETIC 2 Thus, I determined the AQ, D1, pulse value, mixing time, SW and NS, ensuring both the 5xT1 and the proper S/N ratio. The experiments were carried out in BBI and without spin. In general it would be: extraction->exploratory NMR-PCA analysis-> characterization -> determination of T1's -> Quantitative NMR experiments -> Quantification via ERETIC 2 -> SIMCA and PCR.

If you would like to know any additional information, I would be happy to share it with you.